Gel extraction kit qiagen manual

The QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to mg slices) or enzymatic reactions. DNA ranging from 70 bp to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μwww.doorway.rug capacity: 10 µg.  · QIAquickВ® Gel Extraction Kit www.doorway.ru gel extraction www.doorway.ru 10 kb qiaquick gel extraction kit dna cleanup and concentration 3 manual solutions automated solutions, the qiaquick pcr purification kit provides spin columns, are the columns of the qiaquick pcr purification-, gel extraction-, qiaquick gel Estimated Reading Time: 8 mins. QIAquick Gel Extraction Kits (50) () Catalog No. QIAquick Spin Columns 50 Buffer QG* 2 x 50 ml 2 x ml Buffer PE (concentrate) 2 x 10 ml 2 x 50 ml QIAGEN kit and kit component. CAUTION: DO NOT add bleach or acidic solutions directly to the sample-preparation waste.

PCR Purification Kit Gel Extraction Kit Reaction Cleanup Kit Maximum binding capacity: 5 µg 5 µg 5 µg Recovery of DNA: 80% 80% 80% (70 bp – 4kb) (70 bp – 4 kb) (70 bp – 4 kb) Maximum weight of gel slice: mg — Elution volume: 10 µl 10 µl 10 µl Volume of eluate: 9 µl 9 µl 9 µl Capacity of column reservoir: µl µl. The QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to mg slices) or enzymatic reactions. DNA ranging from 70 bp to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl. The QIAEX II and QIAquick Gel Extraction Kit can be used to extract DNA from polyacrylamide gels. The QIAEX II Handbook contains a protocol for Polyacrylamide Gel Extraction. A specialized User-Developed Protocol (QQ05) is available when using the QIAquick Gel Extraction Kit for this purpose.

Silica columns from PCR purification and gel extraction kits are fresh columns from a PCR purification kit (Qiagen) were initially used. This protocol works best with fragments excised from 1% gels, I have extracted from gels Gel Extraction Protocol (QIAquick gel extraction Kit Protocol). Centrifuge at rpm for 1 minute. Go directly to sequencing or re-amplify using a regular PCR protocol to concentrate product. Page 6.