Gateway pdonr vectors manual

Gateway ® Entry Vectors creation of entry clones. For rapid TOPO A variety of Gateway ® entry vectors are available from Invitrogen to facilitate ® Cloning of PCR products, we recommend using the pENTR/D-TOPO® or pENTR/SD/D-TOPO ® Cloning Kits. For traditional restriction enzyme digestion and ligase-mediated cloning, use one of the other pENTR ™ vectors. Gateway Clonase Manual The Donor Vector box, the Gateway® BP Clonase®II Enzyme Mix and Reagents box, and the M13 Sequencing Primers box (Boxes 1–4) contain the following items. Store the Gateway® BP Clonase®II enzyme mix at −30°C to −10°C for up to 6 months. For long-term storage, store at −85°C to −68°C. Page 3/5. Gatewayfi pDONRŽ Vectors This manual is supplied with the following vectors: Product Catalog no. pDONRŽ pDONRŽ pDONRŽ pDONRŽ/Zeo Shipping and Storage pDONRŽ, pDONRŽ, and pDONRŽ are shipped at room temperature. Upon receipt, store at °C. pDONRŽ/Zeo is shipped on blue ice. Upon receipt, storeFile Size: KB.

Gateway® Entry Vectors The MultiSite Gateway® Three-Fragment kit provides the pDONR™ vector to facilitate creation of attL1 and L2-flanked entry clones. Alternatively, a variety of Gateway® entry vectors are available from Life Technologies to allow creation of entry clones using TOPO® Cloning or restriction digestion and ligation. For more. Gateway® pDONR™ Vectors This manual is supplied with the following vectors: Product Catalog no. pDONR™ pDONR™ pDONR™/Zeo Shipping and Storage pDONR™ and pDONR™ are shipped at room temperature. Upon receipt, store at °C. pDONR™/Zeo is shipped on blue ice. Upon receipt, store. P sites found in pDONR ™ First-generation sites were further modified to improve recombination efficiency and resulted in the second-generation. att. P sites found in pDONR ™ and pDONR ™ /Zeo. For more information on characteristics of. att. sites, refer to the Gateway ® Technology with Clonase II manual.

26 янв. г. We review the various types of Gateway destination vectors that are are described in the Gateway cloning manual(s) available from. This Gateway Cloning instruction manual reviews: Recombination Reactions of BP CLONASE pDONR™ with BP CLONASE™ into Entry Vector Mix into pDONR 17 июл. г. Empty Gateway plasmids to start with are called pDONR vectors. We are using Primers with attB flanking sequences (see Gateway manual).